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Dr.Mudasir Rashid
APID: 2854
capPostdoctoral researcher and Instructor at HOWARD UNIVERSITY HOSPITAL Washington, DC
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My Biography

I have extensive expertise in Cancer Biology, More Epigenetics, Biotechnology, Microbiome. I hold a Master of Science (MS) in Biotechnology from the University of Bangalore, India, and a Doctor of Philosophy (PhD) in life science with a focus on Cancer Biology, specifically Gastric Adenocarcinoma.
Currently, I am working in Dr. Ashktorab’s laboratory as a Postdoctoral Researcher in Department of Medicine, Gastroenterology Division, Howard University Hospital Cancer Center, Washington DC, USA.
During my doctoral studies, I focused on understanding the molecular profiles of histone H3 isoforms and variants in gastric adenocarcinoma and exploring novel pathways and potential therapeutic targets. At Howard University, I have conducted exome next-generation sequencing (NGS) on African American colorectal cancer (CRC) patients, leading to the identification of novel and deleterious MSH3 missense mutations, providing insights into their significance in AA-CRC pathogenesis.

Area Of Expertise

Epi-genetic Techniques: Bisulfite conversion method, Methylated DNA Immunoprecipitation (MeDIP), HAT and HDAC assay. • Molecular Biology Techniques: Droplet Digital PCR (ddPCR), Real time-PCR. • Cell Culture and Cancer Biology Techniques: Handling mammalian cell cultures, Transfection, Luciferase assay, Proliferation assay (MTTs and Clonogenic assay), Migration/ Invasion assay (Wound healing, Boyden Chamber). • Analytical and Immunological Techniques: SDS-PAGE, AUT-PAGE, Western-blotting, Co-immunoprecipitation (Co-IP), Immunofluorescence (IF), Immunohistochemistry (IHC) and Micrococcal Nuclease assay (MNase), Comet assay. • Microbiology: Competent cell preparation, transformation, screening and plating, cloning (primer designing), site-directed mutagenesis (SDM), and Protein purification (using combination of ion exchange, size exclusion, and affinity chromatography techniques). Knock-in point-mutation using CRISPR-Cas9 HDR based methods. • RNA seq analysis: RNA isolation, cDNA synthesis, library preparation, adapter ligation, and data analysis.

Area of Intrest

Epigenetics, Microbiome, Cancer biology-Colorectal cancer and saffron

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Fuel properties tested on VCR engine and analysed thoroughly

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Sangeeta Kakkar

Sangeeta Kakkar is an assistant professor of clinical psychology at Shoolini University and a Read More Research Scholar. Delhi is where she obtained her bachelor’s degree in psychology and her postgraduate degree in clinical psychology. Kakkar is a committed teacher who is passionate about learning how previous trauma affects people’s lives and actions.
Kakkar had clinical experience through internships at several hospitals and healthcare facilities before beginning her academic career. She was exposed to a range of mental health issues during her three months as an intern psychologist at Lok-Nayak Jai Prakash Hospital in Delhi Gate, India. Kakkar also worked as an intern in Fortis Healthcare Limited’s Department of Mental Health and Behavioural Sciences, where she worked with children with Autism Spectrum Disease.
Kakkar’s academic achievements are her B.Ed. and qualified UGC-NET Assistant Professor test. Additionally, she was successful on both the Punjab State Teacher Eligibility Test and the Central Teacher Eligibility Test. Kakkar is now conducting research on the stress, coping mechanisms, and mental health issues that parents of children with developmental impairments encounter.
Till now I have published research articles in International peer review journals. Looking forward to working more in the field of clinical psychology.

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