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Dr. Mudasir Rashid
APID: 2854
cap

Post Doctoral Researcher at HOWARD UNIVERSITY HOSPITAL Washington, DC

Expertise

Epi-genetic Techniques: Bisulfite conversion method, Methylated DNA Immunoprecipitation (MeDIP), HAT and HDAC assay. • Molecular Biology Techniques: Droplet Digital PCR (ddPCR), Real time-PCR. • Cell Culture and Cancer Biology Techniques: Handling mammalian cell cultures, Transfection, Luciferase assay, Proliferation assay (MTTs and Clonogenic assay), Migration/ Invasion assay (Wound healing, Boyden Chamber). • Analytical and Immunological Techniques: SDS-PAGE, AUT-PAGE, Western-blotting, Co-immunoprecipitation (Co-IP), Immunofluorescence (IF), Immunohistochemistry (IHC) and Micrococcal Nuclease assay (MNase), Comet assay. • Microbiology: Competent cell preparation, transformation, screening and plating, cloning (primer designing), site-directed mutagenesis (SDM), and Protein purification (using combination of ion exchange, size exclusion, and affinity chromatography techniques). Knock-in point-mutation using CRISPR-Cas9 HDR based methods. • RNA seq analysis: RNA isolation, cDNA synthesis, library preparation, adapter ligation, and data analysis.

Area of Interest

Epigenetics, Microbiome, Cancer biology-Colorectal cancer and saffron

Short Biography

I have extensive expertise in Cancer Biology, More

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Last updated: 2022-06-15

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