Dr. Sworna kumari Chithiraivelu
APID: 9231
capGraduate Student at Tamilnadu veterinary and animal sciences University Chennai, Tamilnadu
 

Expertise


Molecular biology

Area of Interest


Mab Production | DNA VACCINE -IBD Virus | Bioplastic -Microsphere Encapsulation |cloning and expression

Short Biography


• Molecular Biology -DNA vaccine development, More Cloning and expression of recombinant proteins, ELISA, Monoclonal antibody production-screening, Handled Bioreactor/ fermenter, Lyophilizers, Real Time PCR, microsphere preparation and in vitro controlled drug release, Latex agglutination test, Lateral flow assay, Electrophoresis, Immunoelectrophoresis • Cell culture work- Sub culturing cell line like VERO, CHO, BHK and MDBK, Cell Viability, Invitro cytotoxicity assay, Lymphocyte Proliferation Assay, TCID, Cell Cycle analysis by BrDU, transfection, infection of viruses, Cryopreservation, and reviving • Animal handled- Balb/c Mice, Chickens (SPF Eggs) • Protein biochemistry -SDS PAGE Western Blotting, Protein purification, Dialyzing the protein • Microbiology work-Isolation and Identification of Micro-organisms, Staining Techniques, Plant Biotechnology work (Genetic Modification and budding plants/plant products) • Bioinformatics- Databases, Sequence alignment, Primer designing, sequence submission, Phylogenetic Tree construction, Insilico-PCR • Embryo biotechnology- Oocyte collection, Invitro maturation of oocytes, Invitro fertilization, assessment, grading, Embryo biopsy collection using micromanipulator, ICSI, Sexing of embryos by PCR, Cryopreservation

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