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Dr.Mudasir Rashid
APID: 10924
capPostdoctoral researcher and Instructor at HOWARD UNIVERSITY HOSPITAL Washington, DC
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My Biography

I have extensive expertise in Cancer Biology, More Epigenetics, Biotechnology, Microbiome. I hold a Master of Science (MS) in Biotechnology from the University of Bangalore, India, and a Doctor of Philosophy (PhD) in life science with a focus on Cancer Biology, specifically Gastric Adenocarcinoma.
Currently, I am working in Dr. Ashktorab’s laboratory as a Postdoctoral Researcher in Department of Medicine, Gastroenterology Division, Howard University Hospital Cancer Center, Washington DC, USA.
During my doctoral studies, I focused on understanding the molecular profiles of histone H3 isoforms and variants in gastric adenocarcinoma and exploring novel pathways and potential therapeutic targets. At Howard University, I have conducted exome next-generation sequencing (NGS) on African American colorectal cancer (CRC) patients, leading to the identification of novel and deleterious MSH3 missense mutations, providing insights into their significance in AA-CRC pathogenesis.

Area Of Expertise

Epi-genetic Techniques: Bisulfite conversion method, Methylated DNA Immunoprecipitation (MeDIP), HAT and HDAC assay. • Molecular Biology Techniques: Droplet Digital PCR (ddPCR), Real time-PCR. • Cell Culture and Cancer Biology Techniques: Handling mammalian cell cultures, Transfection, Luciferase assay, Proliferation assay (MTTs and Clonogenic assay), Migration/ Invasion assay (Wound healing, Boyden Chamber). • Analytical and Immunological Techniques: SDS-PAGE, AUT-PAGE, Western-blotting, Co-immunoprecipitation (Co-IP), Immunofluorescence (IF), Immunohistochemistry (IHC) and Micrococcal Nuclease assay (MNase), Comet assay. • Microbiology: Competent cell preparation, transformation, screening and plating, cloning (primer designing), site-directed mutagenesis (SDM), and Protein purification (using combination of ion exchange, size exclusion, and affinity chromatography techniques). Knock-in point-mutation using CRISPR-Cas9 HDR based methods. • RNA seq analysis: RNA isolation, cDNA synthesis, library preparation, adapter ligation, and data analysis.

Area of Intrest

Epigenetics, Microbiome, Cancer biology-Colorectal cancer and saffron

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Madhuri GMG

Hello everyone ,I am interested in Machine Learning

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I am Dr. Sripriya Gopalkrishnan, interested in developing AI driven systems to improve patient care.

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Dr. Sripriya Gopalkrishnan

Hi,
Evidence based practice is the need of the hour. Nurses are at the fore front of the health care Read More system and the most important element of health care services.
Digital and AI driven interventions by nurses are now required to enhance patient satisfaction with nursing care and health care outcomes
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Mohammed Bouskout

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I’ve come to this APID with the hope of providing extensive Read More support to the scientific community, particularly in the areas of agrosciences, biotechnologies, and ecology.
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Vivek Kumar

My areas of work includes renewable energy, sustainability, particulate separation, CFD with Read More relevant publications in prestigious journals.
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Nkhensani Mabunda

Hallo everyone I am novice researcher interested in mental health

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Nkhensani Mabunda

Nkhensani Florence Mabunda is a Psychiatric Nursing Science lecturer. Psychiatric Nursing Science is Read More facilitating teaching and learning at Sefako Makgatho Health Science University. Her current and previous research interests focus on mental health care and willing to read and review manuscripts related to mental health and their families.
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