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Dr.Mudasir Rashid
APID: 10924
capPostdoctoral researcher and Instructor at HOWARD UNIVERSITY HOSPITAL Washington, DC
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My Biography

I have extensive expertise in Cancer Biology, More Epigenetics, Biotechnology, Microbiome. I hold a Master of Science (MS) in Biotechnology from the University of Bangalore, India, and a Doctor of Philosophy (PhD) in life science with a focus on Cancer Biology, specifically Gastric Adenocarcinoma.
Currently, I am working in Dr. Ashktorab’s laboratory as a Postdoctoral Researcher in Department of Medicine, Gastroenterology Division, Howard University Hospital Cancer Center, Washington DC, USA.
During my doctoral studies, I focused on understanding the molecular profiles of histone H3 isoforms and variants in gastric adenocarcinoma and exploring novel pathways and potential therapeutic targets. At Howard University, I have conducted exome next-generation sequencing (NGS) on African American colorectal cancer (CRC) patients, leading to the identification of novel and deleterious MSH3 missense mutations, providing insights into their significance in AA-CRC pathogenesis.

Area Of Expertise

Epi-genetic Techniques: Bisulfite conversion method, Methylated DNA Immunoprecipitation (MeDIP), HAT and HDAC assay. • Molecular Biology Techniques: Droplet Digital PCR (ddPCR), Real time-PCR. • Cell Culture and Cancer Biology Techniques: Handling mammalian cell cultures, Transfection, Luciferase assay, Proliferation assay (MTTs and Clonogenic assay), Migration/ Invasion assay (Wound healing, Boyden Chamber). • Analytical and Immunological Techniques: SDS-PAGE, AUT-PAGE, Western-blotting, Co-immunoprecipitation (Co-IP), Immunofluorescence (IF), Immunohistochemistry (IHC) and Micrococcal Nuclease assay (MNase), Comet assay. • Microbiology: Competent cell preparation, transformation, screening and plating, cloning (primer designing), site-directed mutagenesis (SDM), and Protein purification (using combination of ion exchange, size exclusion, and affinity chromatography techniques). Knock-in point-mutation using CRISPR-Cas9 HDR based methods. • RNA seq analysis: RNA isolation, cDNA synthesis, library preparation, adapter ligation, and data analysis.

Area of Intrest

Epigenetics, Microbiome, Cancer biology-Colorectal cancer and saffron

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My research focuses on cancer immunology/immunotherapy.

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Fernando Antonio da Silva Fernandes

Production of new materials using waste to promote circular economy and environmental sustainability

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Fernando Antonio da Silva Fernandes

Production of new materials using waste to promote circular economy and environmental sustainability

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VISWANADHA KUMAR

My recent research proposal on the adaptive reuse of heritage buildings in Visakhapatnam makes a Read More difference in achieving one of the sustainable development goals.
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Sakineh Hargol Zadeh

Lack of potable water is a global challenge today, on the other hand, pathogenic bacteria cause Read More pollution of water resources and cause diseases and in many cases, death. Therefore, quick, accurate, and timely identification of this pathogenic factor is very important. Nowadays, biosensors have received much attention due to their simplicity, high speed, and inexpensive, and they have a promising perspective for controlling water quality and maintaining public health.
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Sakineh Hargol Zadeh

Lack of potable water is a global challenge today, on the other hand, pathogenic bacteria cause Read More pollution of water resources and cause diseases and in many cases, death. Therefore, quick, accurate, and timely identification of this pathogenic factor is very important. Nowadays, biosensors have received much attention due to their simplicity, high speed, and inexpensive, and they have a promising perspective for controlling water quality and maintaining public health.
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